GSM5575501: D12 HiChIP GATA1 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GATA1
Cell type
Cell type Class
Blood
Cell type
CD34+
NA
NA
Attributes by original data submitter
Sample
source_name
D12_HiChIP_GATA1
tissue
cord blood
cell type
Day12 CD34+ cells
antibody
GATA1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell populations of distinct differentiation stages were isolated by the following cell surface markers: Pro-E cells are CD235a+CD71+CD45lowCD36+CD117highCD105high; Baso-E cells are CD235a+CD71+CD45lowCD36+CD117dimCD105dim; Poly-E cells are CD235a+CD71+CD45lowCD36+CD117-CD105-FSChigh; Ortho-E cells are CD235a+CD71+CD45lowCD36+CD117-CD105-FSClow. Day-12 erythroblasts generated from ex vivo CD34+ erythroid differentiation culture were used. The library was prepared using the Tn5 transposome DNA library preparation kit (Vazyme, Cat.# TD502) as per the manufacturer's protocol. DNA was then subjected to double-size selection using DNA Clean Beads (Vazyme, Cat. # N411) in order to isolate fragments between 300 and 600bp.