Antigen

Antigen Class

TFs and others

Antigen

GATA1

Cell type

Cell type Class

Blood

Cell type

CD34+

NA

NA

Sample

source_name

D12_HiChIP_GATA1

tissue

cord blood

cell type

Day12 CD34+ cells

antibody

GATA1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell populations of distinct differentiation stages were isolated by the following cell surface markers: Pro-E cells are CD235a+CD71+CD45lowCD36+CD117highCD105high; Baso-E cells are CD235a+CD71+CD45lowCD36+CD117dimCD105dim; Poly-E cells are CD235a+CD71+CD45lowCD36+CD117-CD105-FSChigh; Ortho-E cells are CD235a+CD71+CD45lowCD36+CD117-CD105-FSClow. Day-12 erythroblasts generated from ex vivo CD34+ erythroid differentiation culture were used. The library was prepared using the Tn5 transposome DNA library preparation kit (Vazyme, Cat.# TD502) as per the manufacturer's protocol. DNA was then subjected to double-size selection using DNA Clean Beads (Vazyme, Cat. # N411) in order to isolate fragments between 300 and 600bp.

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

47198400

Reads aligned (%)

45.2

Duplicates removed (%)

11.9

Number of peaks

3874 (qval < 1E-05)

hg19

Number of total reads

47198400

Reads aligned (%)

45.1

Duplicates removed (%)

12.0

Number of peaks

3707 (qval < 1E-05)